Figure 3.
αOGTKOmice exhibit reduced fed and fasting glucagon levels and decreased hepatic glucose output but remain euglycemic under fed and fasted conditions. Fasting experiment conducted in 4-month-old male mice (A) for 24 h (n = 10 per group) and in 4-month-old female mice (B) for 16 h (n = 6 per group). Circulating plasma glucagon levels in 4-month-old male mice in duration of the fasting experiment from “A” at timepoint 0 and 24 (C) and in 4-month-old female mice in duration of the fasting experiment from “B” at timepoint 0 and 16 (D). Glucose levels after intraperitoneal injection of pyruvate in 3-month-old males (E), (n = 6 per group) and in 3-month-old females (F), (n = 6 per group). Glucose levels after intraperitoneal injection of glucagon in 3.5-month-old males (G), (n = 6 per group). Liver glycogen assessment in 6-month-old male and female mice (H), (n = 4 per group). Data represent mean ± SEM, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 compared with control. Analysis was done by unpaired, two-tailed Student’s t-tests, repeated measures one-way ANOVA, and two-way ANOVA.