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. 2021 Jan 16;296:100297. doi: 10.1016/j.jbc.2021.100297

Figure 8.

Figure 8

Male αOGTKOmice show reduction in GFP-positive α-CaMKII-positive cells in the hypothalamic PVN. Immunofluorescent staining for α-CaMKII (red), in tandem with endogenous GFP Cre reporter (green) in 4-month-old male mouse fixed coronal brain sections (A). (scale = 50 μm). 10× image of PVN in a 4-month-old Gcg-cre, OGTWT; CAG-GFP mouse, stained for α-CaMKII (red) and NeuN1 (blue), in tandem with endogenous GFP (green) (B). (scale = 200 μm). Immunofluorescent PVN staining of male control and αOGTKO mice for α-CaMKII (red) and NeuN1 (blue), in tandem with endogenous GFP (green) (C, B is reshown in C for a comparison as control). (scale = 200 μm). Full PVN Cre(+) cell number, per mouse brain, was visualized in three adjacent 40 μm sections. Quantification of Cre-positive cell number in PVN, totaled between three concomitant sections per mouse brain (D) (n= 3 per group). Cre-positive cells were only counted if colocalized with both α-CaMKII and NeuN1. Immunofluorescent PVN staining of male control and i-αOGTKO mice for α-CaMKII (green) and NeuN1 (blue), in tandem with endogenous RFP (red) (E). (scale = 200 μm). Data represent mean ± SEM, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 compared with control. Analysis was done by unpaired, two-tailed Student’s t-tests.