Figure 4.

Qualitative assessment of agonist-promoted β-arrestin2 recruitment by WT and mutant TAS2R14 receptors. WT and IL-5A receptors exposed to agonist (10 min) displayed a redistribution of β-arrestin2–GFP from the cytosol to the cell membrane and a punctate accumulation pattern at the cell membrane. This appearance was not observed with CT-5A and IL/CT-10A receptors. Shown is a representative of five experiments performed. CT, carboxy-terminal tail; DPD, diphenhydramine; IL, intracellular loop; TAS2R14, bitter taste 2 receptor member 14.