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Proximity ligation assay (PLA) reveals differential β-arrestin2 binding to WT and mutant receptors.A, representative experiment showing agonist-promoted β-arrestin2 binding (red signal) with the indicated receptors. B, mean ± SD, and individual results, from five PLA experiments. ∗p < 0.01 versus WT; #not significant versus 0% change as determined by the one-sample t test. DAPI, 4′,6-diamidino-2-phenylindole; DPD, diphenhydramine; IL, intracellular loop.
