Table 1.

Desensitization of TAS2R14

Plasmid/siRNA	Slope (N = 5)	p Versus WT/siCtrl
HEK-293T cells
WT	−1.51 ± 0.19	REF
IL/CT-10A	−0.22 ± 0.23	<0.003
CT-5A	0.44 ± 0.34	<0.002
IL-5A	−0.99 ± 0.23	0.127
HASM cells
SiCtrl	−1.74 ± 0.03	REF
siGRK2	−0.03 ± 0.35	<0.001

Transfected HEK-293T cells (upper section) expressing the indicated receptors were exposed to agonist and [Ca²⁺]_i monitored in real time. In the lower section, the same experiments were performed using HASM cells, which were transfected with siRNA constructs targeting GRK2 (siGRK2) or scrambled control (siCtrl). The slopes of the responses from 10 to 50 s were determined by linear regression and compared with that of WT in the case of the HEK-293 experiments or siCtrl in the case of HASM cells. REF is the reference for statistical comparison.