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. 2021 Jan 20;296:100314. doi: 10.1016/j.jbc.2021.100314

Figure 1.

Figure 1

Radil is a potential Ras-interacting protein.A, coomassie Brilliant Blue staining of flag pulldown. HEK293T cells transfected with plasmid expressing HRasV12 tagged with Flag or with the empty vector (CT) for 24 h, after which cell lysates were collected for affinity-pulldown using Flag M2 agarose as described in Experimental procedures and Supplemental Procedures. Affinity-purified proteins were subjected to SDS-PAGE analysis. Arrow indicates the position of Radil or HRas. The single star (∗) indicates IgG heavy-chain band. The double stars (∗∗) indicate IgG light-chain band. B, affinity-purified proteins were subjected to mass spectrometric analysis. Proteins identified with top scores were listed and Radil was highlighted. C, a survey of Radil expression in various tumor cell lines via Western blotting. The arrow indicates the Radil specific bands. The star (∗) denotes nonspecific bands. IgG, immunoglobulin G.