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. 2021 Jan 20;296:100314. doi: 10.1016/j.jbc.2021.100314

Figure 2.

Figure 2

Radil physically interacts with KRas.A, HEK293T cells transfected with Flag-Radil were lysed, and cell lysates were immunoprecipitated with the anti-Flag antibody or control IgG. Flag immunoprecipitates, along with lysate inputs, were blotted with antibodies for Flag, KRas 4b, and Pan-Ras proteins. B, HEK293T cells were transfected with the plasmid expressing Flag-KRasV12 for 24 h, after which cell lysates were immunoprecipitated with the anti-Flag antibody. Flag immunoprecipitates, along with lysate inputs, were blotted for Radil and Flag. C, domain structures of full-length Radil (FL) and its mutant without RA domain (RAΔ). D, HEK293T cells transfected with FLAG-Radil FL and RAΔ expression plasmids for 24 h, after which cell lysates were immunoprecipitated with the anti-Flag antibody. Flag immunoprecipitates, along with lysate inputs, were blotted for KRas4B, Rap1, and Flag. IgG, immunoglobulin G.