Figure 5.

Radil mediates growth factor–induced activation of cRaf and MEK. A549 cells were transfected with Radil siRNAs (siRadil) or nontargeting control pool siRNAs (siControl) for 24 h, starved in low (0.2%) FBS medium for 16 h, and then fed 20% serum for various times as indicated. Cell lysates were then blotted for Radil, phospho-cRaf 338, phospho-MEK, and actin. Signals of p-cRaf and p-MEK were quantified. The arrow indicates the position of Radil. The values presented are derived from the density of phospho-form normalized by the density of actin. ∗ indicates nonspecific bands. FBS, fetal bovine serum.