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. 2021 Jan 20;296:100314. doi: 10.1016/j.jbc.2021.100314

Figure 6.

Figure 6

Radil positively regulates cell proliferation, migration, and EMT.A, A549 cells transfected with Radil siRNAs (siRadil), KRas siRNAs (siKRas), and combination of Radil and KRas siRNAs (siR+K) for various times as indicated. Control cells were transfected for the same length of time with nontargeting control pool siRNAs (siControl). Cell proliferation was measured as described in Experimental procedure. B, A549 cells transfected with Radil siRNAs and/or KRas siRNAs for 24 h. Cell lysates were then blotted for Radil, KRas, E-cadherin, Vimentin, Zeb1, ZO-1, Snail, and actin. Signals of E-cadherin, vimentin, ZEB1, ZO-1, and Snail were quantified by densitometry. The values presented are derived from the density of each protein normalized by the density of actin. C, representative images of transwell invasion assays. A549 cells transfected with Radil siRNAs and/or KRas siRNAs for 24 h were seeded in Matrigel for cell-invasion assay. Cells transfected with nontargeting control pool siRNAs (siControl) were used as control. Scale bar = 200 μm. D, quantification of transwell invasion assays (top panel) and total counts of invaded cells in three individual images from transwell inserts (bottom panel). Data on cell invasion after various treatments were summarized and quantified. Data represent the mean (±standard deviation, SD) of three independent experiments, each performed in triplicate and are presented relative to control. Error bars indicate SDs. Stars indicate statistical significance at p < 0.05, ∗; and p < 0.01, ∗∗. EMT, epithelial–mesenchymal transition.