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. 2021 Jan 20;296:100318. doi: 10.1016/j.jbc.2021.100318

Figure 3.

Figure 3

20E-induced dephosphorylation of INSRβ was repressed by cycloheximide and a phosphatase inhibitor cocktail.A, the effect of cycloheximide on 20E-induced dephosphorylation of INSRβ in HaEpi cells (50 μM for 1 h). B, the effect of a phosphatase inhibitor cocktail on 20E-induced dephosphorylation of INSRβ in HaEpi cells (1% A and 1% B cocktail for 30 min). 12.5% gel. C and D, statistical analysis of (A) and (B) using ANOVA, different letters represented significant differences (p < 0.05). The bars indicate the mean ± SD of three times repetition. ImageJ software was used to transform the image data.