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. 2021 Jan 20;296:100318. doi: 10.1016/j.jbc.2021.100318

Figure 5.

Figure 5

PTEN is involved in 20E-induced dephosphorylation of INSRβ. A, qRT-PCR showing the expression profiles of Pten in various tissues during development, with Actb as the quantity and quality control. B, western blotting showing the effect of knockdown of Pten or overexpression of Pten in HaEpi cells on INSRβ; 12.5% SDS-PAGE. C, statistical analysis of (B) using ANOVA, different letters represented significant differences (p < 0.05). The bars indicate the mean ± SD of three times repetition. ImageJ software was used to transform the image data.