Figure 9.

20E increased hemolymph glucose levels during metamorphosis.A, the hemolymph glucose levels during development. The error bars were calculated from three independent repeats. B and C, the increase of the hemolymph glucose levels by 20E induction in dose and time. DMSO was used as the solvent control. D, the hemolymph glucose levels detection after the last injection of dsRNA for 24 h, 48 h, 72 h. Statistical analysis was conducted using ANOVA for (B), different letters represented significant differences (p < 0.05). Statistical analysis of the data in C and D by Student’s t-test from three biological repeats (∗p < 0.05; ∗∗p < 0.01). The bars indicate the mean ± SD of three times repetition.