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. 2021 Jan 23;296:100329. doi: 10.1016/j.jbc.2021.100329

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© 2021 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

AltB2R and B2R interaction shown by bimolecular fluorescence complementation (BiFC) in HeLa cells.A, expression of B2R^Flag-VN and VC-AltB2R^HA results in BiFC signals in transfected HeLa cells. BiFC signals is lost when competing B2R^Flag or AltB2R^HA interacts with available pool of VC-AltB2R^HA or B2R^Flag-VN, respectively. B, no detectable BiFC signals from nonspecific assembly of the Venus protein. Scale bar: 10 μm. A and B, representative single confocal mid-section images from three independent experiments are shown.