Table 3.
Equilibrium dissociation constant (KD) values determined for wildtype and N34S variant SPINK1 inhibitors against nonsulfated and sulfated human trypsins purified from the conditioned medium of transfected HEK 293T cells
| KD (pM) | Hu1 (HEK 293T) | Hu1-SO4 (HEK 293T) | Hu2 (HEK 293T) | Hu2-SO4 (HEK 293T) |
|---|---|---|---|---|
| SPINK1 wildtype | 4.4 ± 0.4 | 40.2 ± 7.8 | 0.5 ± 0.2 | 9.0 ± 1.1 |
| SPINK1 N34S | 4.7 ± 0.3 | 37.5 ± 4.3 | 0.8 ± 0.2 | 6.3 ± 0.7 |
Hu1, nonsulfated human cationic trypsin; Hu2, nonsulfated human anionic trypsin; Hu1-SO4, sulfated human cationic trypsin; Hu2-SO4, sulfated human anionic trypsin.
Measurements were carried out as described under Experimental procedures section. Data from three experiments were fitted globally. The error of the fit is indicated.