Table 5.
Equilibrium dissociation constant (KD) values determined for wildtype SPINK1 and seven variants on sulfated cationic and anionic trypsin isoforms
| KD (pM) | Hu1-SO4 | Hu2-SO4 |
|---|---|---|
| SPINK1 wildtype | 62.2 ± 10.3 | 36.7 ± 2.3 |
| SPINK1 N34S | 32.3 ± 2.9 | 16.7 ± 0.8 |
| SPINK1 N37S | 86.9 ± 1.0 | 21.4 ± 1.9 |
| SPINK1 I42M | 200 ± 42 | 258 ± 32 |
| SPINK1 P55S | 101 ± 20 | 123 ± 26 |
| SPINK1 R65Q | 59.2 ± 9.4 | 6.0 ± 2.5 |
| SPINK1 Q68R | 68.0 ± 7.1 | 27.0 ± 0.6 |
| KD(μM) | ||
| SPINK1 K41N | 1.29 ± 0.07 | 1.15 ± 0.03 |
Hu1-SO4, sulfated human cationic trypsin purified from pancreatic juice; Hu2-SO4, sulfated human anionic trypsin purified from pancreatic juice.
Measurements were carried out as described under Experimental procedures section. Data from three experiments were fitted globally. The error of the fit is indicated.