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. 2021 Feb 2;296:100364. doi: 10.1016/j.jbc.2021.100364

Figure 6.

Figure 6

Pharmacological inhibition of KCC2 with 11K stimulates apoptosis in neuronal cultures.A, immunoblots of DIV18-DIV21 cortical/hippocampal neuronal lysates treated with DMSO or 1 μM 11K for 10, 30, or 60 min were probed for the total and cleaved expression of the extrinsic proapoptotic marker; caspase 8, the intrinsic proapoptotic markers; caspase 3 and caspase 7, PARP, KCC2, and β-actin. B, the bar charts show the quantification of the levels of proapoptotic markers cleaved caspase 8, cleaved caspase 3, and cleaved PARP, with significantly changed expression marked (∗p < 0.05, ∗∗p < 0.01, n = 3 replicates). C, immunoblots of DIV18–21 cortical/hippocampal neuronal lysates with 500 nM TTX alone or TTX+11K for 10, 30, or 60 min were probed for expression of total and cleaved caspase 3. D, the bar charts show the quantification of total and cleaved caspase 3 expression.