Table 3.
Three days precision and accuracy of the HPLC method for quantification of CoQ10
| Standard | Cnom, µg/mL | Interday variability, n = 15 | Intraday variability, n = 9 | ||||
| mean±SD, µg/mL | CV, % | RE, % | mean±SD, pg/mL | CV, % | RE, % | ||
|---|---|---|---|---|---|---|---|
| LLOQ | 0.0618 | 0.062±0.002 | 2.5 | −0.1 | 0.061±0.002 | 2.8 | −1.2 |
| QC1 | 0.1854 | 0.187±0.004 | 1.9 | 1.1 | 0.187±0.003 | 1.7 | 0.6 |
| QC2 | 4.12 | 4.223±0.069 | 1.6 | 2.5 | 4.224±0.016 | 0.4 | 2.5 |
| QC3 | 8.24 | 8.532±0.149 | 1.8 | 3.5 | 8.643±0.042 | 0.5 | 4.9 |
QC1, 3 times the LLOQ; QC2 medium; QC3, 75% of the upper calibration curve range; SD, standard deviation; COQ10, coenzyme Q10.