Table 1.
Gradient elution program during the chromatographic analysis
| Time | Mobile phase A% | Mobile phase B% |
|---|---|---|
| 0.0 | ||
| 0.0 | 98.0 | 2.0 |
| 0.1 | 98.0 | 2.0 |
| 1.5 | 98.0 | 2.0 |
| 2.0 | 80.0 | 20.0 |
| 3.0 | 60.0 | 40.0 |
| 7.4 | 40.0 | 60.0 |
| 7.5 | 2.0 | 98.0 |
| 8.0 | 2.0 | 98.0 |
| 9.0 | 98.0 | 2.0 |
| 11.0 | 98.0 | 2.0 |
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