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. 2021 Mar 10;9(3):e001946. doi: 10.1136/jitc-2020-001946

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See https://creativecommons.org/licenses/by/4.0/.

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Biological effect of the interaction between tumor-associated neutrophils (TANs) and tumor-associated macrophages (TAMs) on intrahepatic cholangiocarcinoma (ICC) cells. (A) CCK8 cell proliferation assay showed that conditioned medium (CM) from TAN and TAM promoted ICC cell proliferation. Data are shown as the mean±SD (n=6 for each group). (B) Invasion of ICC cells cultured with CM from TAN and TAM compared with that of control ICC cells. The graphs show the number of invasive cells after 48 hours. Data are shown as the mean±SD (n=6 for each group). (C) Cultured with CM from TAN and TAM increased the colony-formation activity of ICC cells Data are shown as the mean±SD (n=6 for each group). *P<0.05, ***p<0.001.