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Immunohistochemistry of TLR7 in wound samples was carried out in a similar fashion to that of TLR9. Upper panel shows the negative (left) and positive (right) staining. In the negative control, serum and secondary antibodies were applied but no primary TLR7 antibody was added to the staining solution to check the non‐specific binding of the primary antibody. Lower panel shows the immunohistochemistry for TLR7 in non‐diabetic control wound (left) and diabetic wound (right).
