Skip to main content
. 2015 Jan 14;13(5):927–935. doi: 10.1111/iwj.12411

Figure 7.

IWJ-12411-FIG-0007-b

Western blot assay showing that protein expression of TLR9 and TLR7 was elevated in diabetic foot ulcer (DFU) patients. Tissue samples collected during debridement process from control (C) and DFU patients were homogenised, and Western blot analysis was performed for expression of TLR9, TLR7 and GAPDH proteins in 43 DFU subjects and 6 controls. Alpha Imager 2200 software version 3.1.2 was used to quantify band density. The relative percent ratios of proteins (toll‐like receptors, TLRs, versus GAPDH) were calculated. Unpaired t‐test was used to check the difference between the mean values of TLR7 and TLR9 proteins in DFU and control subjects using GraphPad Prism. A two‐tailed P value < 0·05 was considered as statistically significant. Bar graph showing up‐regulation of TLR7 and TLR9 proteins in wounds of type 2 diabetes mellitus (T2DM) patients compared with controls (P value ≤ 0·001, t = 4·20 for TLR7 and P value ≤ 0·001, t = 4·27 for TLR9).