Figure 1.

Representative immunopatterns of FN‐fibrin complexes in plasma of PAD patients after revascularisation of ischaemic lower limbs. Blood plasma samples of 142 PAD patients and 37 normal individuals were subjected to SDS‐agarose gel electrophoresis under non‐reducing conditions (25). The bands were transferred to a nitrocellulose membrane (Serva Electrophoresis GmbH, Heidelberg, Germany) and developed with anti‐FN monoclonal antibody (Clone FN30‐8, TaKaRa Shuzo Co. Ltd., Shiga, Japan). Lanes 1 to 2, normal plasma; lanes 2 to 7, PAD patients’ plasma