Skip to main content
. 2017 Mar 29;14(6):993–1005. doi: 10.1111/iwj.12748

Figure 3.

IWJ-12748-FIG-0003-c

Evaluation of hypothermically stored amniotic membrane (HSAM) effects on fibroblasts: (A) quantitative proliferation assay in assay media alone or HSAM conditioned media (CM) in assay media (with 50%, 25% and 10% CM by volume); (B) qualitative analysis of proliferation assay with CFDA (green) and Dapi (blue) staining at 14 days; (C) quantitative analysis of fibroblast migration over 24 hours in growth media, assay media and 50%, 25% and 10% HSAM CM by volume; and (D) confirmatory images of cell migration with cells stained with crystal violet. For quantitative assays, significance compared to assay media is denoted by: *P < 0·05, **P < 0·01, ***P < 0·001 and ****P < 0·0001.