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. 2017 Mar 29;14(6):993–1005. doi: 10.1111/iwj.12748

Figure 4.

IWJ-12748-FIG-0004-c

Evaluation of hypothermically stored amniotic membrane (HSAM) effects on human epidermal keratinocytes (HEKs). (A) Quantitative analysis of proliferation of HEKs cultured under various conditions, including growth media, assay media and HSAM conditioned media (50%, 25% and 10%). Migration of HEKs in response to growth media, assay media and 50%, 25% and 10% HSAM CM evaluated quantitatively (B) and qualitatively with crystal violet staining (C). For quantitative assays, significance compared to assay media is denoted by *P < 0·05, **P < 0·01, ***P < 0·001 and ****P < 0·0001.