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Qualitative evaluation of fibroblast interaction with hypothermically stored amniotic membrane (HSAM) was completed using staining. HSAM was cultured with or without fibroblasts for 2 weeks, and samples were processed and stained with haemetoxylin and eosin (H&E), Masson's trichrome, Alcian blue, or Verhoff's stains. Representative images were taken of H&E (A and B), Masson's trichrome (C and D), Alcian blue (E and F) and Verhoeff's stain (G and H).
