Table 1.
Biofilm analysis and DFU location, wound duration in weeks and whether infected or non‐infected. Presence (+) or absence (−) of biofilm as determined by SEM and FISH analysis, degree of biofilm infection (score) and predominant species identified using massively parallel DNA sequencing
| Patient number | Biofilm (+ or −) | Wound metrics | |||||
|---|---|---|---|---|---|---|---|
| SEM | Score | FISH/PNA‐FISH | Biofilm diversity | Location of DFU | Duration of DFU (weeks) | Infection status | |
| S01 | + | 4 | + | Multi‐species only | Plantar metatarsal head | 8 | Infected |
| S02 | + | 5 | + | Mono‐species and multi‐species | Plantar metatarsal head | 16 | Infected |
| S03 | + | 4 | NS | NS | Plantar metatarsal head | 36 | Infected |
| S04 | + | 4 | + | Mono‐species and multi‐species | Plantar metatarsal head | 14 | Infected |
| S05 | + | 3 | NS | NS | Plantar metatarsal head | 24 | Infected |
| S06 | + | 4 | NS | NS | Fifth toe dorsal | 12 | Infected |
| S07 | + | 4 | + | Multi‐species only | Plantar metatarsal head | 72 | Infected |
| S08 | NS | NS | + | Mono‐species only | Fourth toe dorsal | 6 | Infected |
| S09 | + | 4 | + | Multi‐species only | Second toe apex | 8 | Infected |
| S10 | + | 3 | NS | NS | Heel | 20 | Infected |
| S11 | + | 4 | NS | NS | Heel | 12 | Infected |
| S12 | + | 3 | + | Mono‐species and multi‐species | Plantar metatarsal head | 24 | Infected |
| S13 | + | 4 | + | Mono‐species and multi‐species | Plantar metatarsal head | 6 | Infected |
| S14 | + | 4 | NS | Plantar metatarsal head | 20 | Infected | |
| S15 | NS | NS | + | Multi‐species only | Fourth toe apex | 12 | Infected |
| S16 | NS | NS | + | Mono‐species and multi‐species | Hallux | 8 | Infected |
| S17 | + | 4 | + | Multi‐species only | Hallux | 26 | Infected |
| S18 | + | 4 | + | Mono‐species only | Plantar metatarsal head | 32 | Infected |
| S19 | − | 0 | + | Mono‐species and multi‐species | Hallux | 12 | Infected |
| S20 | + | 3 | + | Multi‐species only | Second toe apex | 16 | Infected |
| S21 | + | 5 | + | Mono‐species and multi‐species | Medial hallux | 8 | Infected |
| S22 | NS | NS | + | Mono‐species and multi‐species | Medial hallux | 18 | Infected |
| S23 | NS | NS | + | Mono‐species and multi‐species | Heel | 12 | Infected |
| S24 | + | 4 | NS | NS | Heel | 24 | Infected |
| S25 | NS | NS | + | Multi‐species only | Hallux apex | 9 | Infected |
| S26 | + | 5 | + | Multi‐species only | Plantar midfoot | 3 | Infected |
| S27 | + | 5 | + | Mono‐species and multi‐species | Plantar metatarsal head | 3 | Infected |
| S28 | + | 5 | NS | NS | Plantar metatarsal head | 6 | Infected |
| S29 | + | 5 | NS | NS | Plantar midfoot | 52 | Infected |
| S30 | + | 4 | NS | NS | Plantar midfoot | 30 | Infected |
| S31 | + | 5 | NS | NS | Plantar metatarsal head | 3 | Infected |
| S32 | + | 5 | NS | NS | Plantar metatarsal head | 5 | Infected |
| S33 | + | 4 | + | Mono‐species and multi‐species | Heel | 5 | Infected |
| S34 | + | 5 | + | Multi‐species only | Heel | 12 | Infected |
| S35 | + | 5 | + | Mono‐species and multi‐species | Heel | 6 | Infected |
| S36 | + | 4 | NS | NS | Plantar metatarsal head | 12 | Infected |
| S37 | + | 4 | NS | NS | Hallux | 9 | Infected |
| S38 | + | 4 | + | Mono‐species and multi‐species | Hallux | 8 | Infected |
| S39 | + | 4 | + | Multi‐species only | Hallux | 12 | Infected |
| S40 | + | 4 | NS | NS | Heel | 72 | Non‐infected |
| S41 | + | 4 | NS | NS | Plantar metatarsal head | 40 | Non‐infected |
| S42 | + | 4 | + | Mono‐species and multi‐species | Heel | 6 | Non‐infected |
| S43 | + | 4 | NS | NS | Plantar metatarsal head | 24 | Non‐infected |
| S44 | NS | NS | + | Mono‐species and multi‐species | Plantar metatarsal head | 12 | Non‐infected |
| S45 | + | 4 | + | Mono‐species and multi‐species | Heel | 36 | Non‐infected |
| S46 | + | 5 | + | Mono‐species and multi‐species | Plantar metatarsal head | 72 | Non‐infected |
| S47 | + | 5 | + | Multi‐species only | Heel | 7 | Non‐infected |
| S48 | − | 1 | + | Multi‐species only | Plantar midfoot | 28 | Non‐infected |
| S49 | + | 4 | + | Multi‐species only | Heel | 18 | Non‐infected |
| S50 | + | 5 | + | Multi‐species only | Heel | 28 | Non‐infected |
| S51 | + | 4 | + | Mono‐species and multi‐species | Plantar metatarsal head | 27 | Non‐infected |
| S52 | + | 5 | + | Mono‐species and multi‐species | Plantar metatarsal head | 28 | Non‐infected |
| S53 | + | 4 | NS | NS | Hallux | 6 | Non‐infected |
| S54 | + | 5 | + | Mono‐species and multi‐species | Hallux | 6 | Non‐infected |
| S55 | + | 4 | + | Mono‐species and multi‐species | Heel | 6 | Non‐infected |
| S56 | + | 3 | + | Multi‐species only | Plantar metatarsal head | 16 | Non‐infected |
| S57 | + | 5 | + | Multi‐species only | Heel | 20 | Non‐infected |
| S58 | + | 5 | + | Mono‐species and multi‐species | Lateral leg | 14 | Non‐infected |
| S59 | + | 4 | + | Multi‐species only | Heel | 10 | Non‐infected |
| S60 | + | 5 | + | Mono‐species and multi‐species | Plantar metatarsal head | 27 | Non‐infected |
| S61 | + | 4 | + | Multi‐species only | Hallux | 8 | Non‐infected |
| S62 | + | 4 | + | Mono‐species and multi‐species | Plantar metatarsal head | 12 | Non‐infected |
| S63 | + | 5 | + | Mono‐species and multi‐species | Hallux | 6 | Non‐infected |
| S64 | + | 5 | NS | NS | Heel | 52 | Non‐infected |
| S65 | + | 5 | + | Mono‐species and multi‐species | Plantar metatarsal head | 9 | Non‐infected |
NS, inability to obtain an additional sample for microscopy from that patient.