Fig. 4.

HDX-MS reveals flexible tethers on ARIH2 and RBX2. HDX-MS shows residues 46 to 58 of ARIH2 are highly exchanging when ARIH2 is alone (green) or bound to the ASB9-CRL (magenta). This segment of ARIH2 (orange) is colored in red in the structural model of the ASB9-CRL (expanded view). Similarly, RBX2 residues 41 to 47 are highly exchanging in the ASB9-ELOB/C-CUL5-RBX2 complex (brown) as well as in the neddylated complex (black). This segment of RBX2 (olive) is also colored red in the structural model of the ASB9-CRL (expanded view).