Fig. 6.

Immunohistochemistry analysis of the five selected differentially expressed proteins. Line chart of the proteomic result, immunohistochemistry grading examples and immunohistochemistry grading result table are presented from left to right for each target protein. Staining was graded according to intensity and percentage of positive cells as follows: 0, no staining or <15% positive cells; 1, weak staining in > 50% of cells, or moderate staining in 15% to 50% of cells; 2, moderate staining in > 50% of cells or strong staining in 15% to 50% of cells; 3, strong staining in >50% of cells.