Fig. 1.

Isolation of the meiotic prophase I spermatocytes.A, experimental design and workflow. B, strategy to obtain the spermatogenesis-synchronized mice. C–G, cross sections of H&E-stained testes obtained from spermatogenesis-synchronized mice on postnatal day 37 (P37) to P46. Roman numerals in each tubule designate the seminiferous tubule stage judging by the criteria described previously (26). The scale bar represents 50 μm. H–L, FACS plots of Hoechst 33342 stained testes cells obtained from the spermatogenesis-synchronized mice, their corresponding seminiferous tubule stages were labeled in C–G. M–T, chromatin spreading of the FACS-sorted spermatocytes that were costained with DAPI (blue), anti-γH2AX (green), and anti-SYCP3 (red). The scale bar represents 5 μm. FACS, fluorescence-activated cell sorting; MACS, magnetic-activated cell sorting; RS, round spermatid.