Fig. 6.

Phenotypic validation of the PDHA2 in meiosis.A, Kyoto Encyclopedia of Genes and Genomes pathways enriched in the top 500 candidate proteins. B, dynamic abundances of the six proteins of the pyruvate dehydrogenase complex. The error bars represent SEM from triplicate samples. C, comparison of the weights of the Pdha2^+/− and Pdha2^−/− mice testes (n = 10, unpaired two-tailed t test, p < 0.0001). D, cross sections of the H&E-stained seminiferous tubules from the 8-week-old Pdha2^+/− (upper panel) and Pdha2^−/− mice (lower panel); the insets denote the specific seminiferous tubules under a higher magnification. The arrow points to a pachytene-like cell. E, cross sections of the H&E-stained epididymis from the 8-week-old Pdha2^+/− (upper panel) and Pdha2^−/− (lower panel) mice. The scale bar represents 50 μm. PDHA2, pyruvate dehydrogenase alpha 2; RS, round spermatid; TCA, tricarboxylic acid.