Fig. 8.

Imaging of homologous recombination and synapsis in the heterozygous and homozygous gene knockout mice.Zcwpw1 (A–D), Tesmin (F–I), and 1700102P08Rik (K–N). Confocal images of the cells coimmunostained with anti-SYCP3 (red) and anti-γH2AX (green) for the display of DSB formation in leptotene (A, F, K) and sex body formation in pachytene (B, G, L), anti-SYCP3 (red) and anti-SYCP1 (green) for the examination of synapsis in pachytene (C, H, M) and anti-SYCP3 (red) and anti-MLH1 (green) for the display of crossover formation in pachytene (D, I, N). The scale bar represents 50 μm. E, J, O, comparison of the MLH1 foci number in spermatocytes derived from Zcwpw1^+/+ and Zcwpw1^−/− (E), Tesmin^−/− and Tesmin^+/− (J), and 1700102P08Rik^+/− and 1700102P08Rik^−/− (O) mice. ∗∗∗∗ represents p < 0.0001 in unpaired two-tailed t test. DSB, double-strand break; n.s., not significant.