Table 2. Primer sequence used for qRT-PCR.
Primer (5ʹ→3ʹ) | ||
Gene | Forward | Reverse |
MyoG | GAGACATCCCCCTATTTCTACCA | GCTCAGTCCGCTCATCGCC |
PGC1alpha | CACCAAACCCACAGAAAACAG | GGGTCAGAGGAAGAGATAAAGTTG |
PGC1beta | TCCTGTAAAAGCCCGGAGTAT | GCTCTGGTAGGGGCAGTGA |
NRF1 | AATGTCCGCAGTGATGTCC | GCCTGAGTTTGTGTTTGCTG |
NRF2 | TGAAGTTCGCATTTTGATGGC | CTTTGGTCCTGGCATCTCTAC |
TFAM | CACCCAGATGCAAAACTTTCAG | CTGCTCTTTATACTTGCTCACAG |
ERRalpha | CCACCCCCTGTTTTGCAT | CTGGCTGCTTGTAGGACACA |
ERRbeta | ACGGCTGGATTCGGAGAAC | TCCTGCTCAACCCCTAGTAGATTC |
PPARbeta | TAGAAGCCATCCAGGACACC | CCGTCTTCTTTAGCCACTGC |
Fkbp5 | TGGTGTTCGTTGTTGGGGAA | AACTTAGGCTTCCCGGCTTC |
Fibin | CCGGGGGAACCTTTTTCCTT | CTGGCATTTCTCGGGGTCAT |
ESR1 | TCCTAACTTGCTCCTGGACAGG | GTAGCCAGCAACATGTCA |
ESR2 | TTCTTTCTCATGTCAGGCACA | CTCGAAGCGTGTGAGCATT |
PLIN2 | CCCGCAACCTGACCCAGCAG | CGCCTGCCATCACCCCCAAG |
FASN | GGAGGTGGTGATAGCCGGTAT | TGGGTAATCCATAGAGCCCAG |
Cox2 | ATAACCGAGTCGTTCTGCCA | GCTTGATTTAGTCGGCCTGG |
18S | CTCAACACGGGAAACCTCAC | CGCTCCACCAACTAAGAACG |
GAPDH | CATCTTCCAGGAGCGAGACC | TGAAGTCGCAGGAGACAACC |