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. 2021 Feb 17;13(4):5804–5823. doi: 10.18632/aging.202505

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Copyright: © 2021 Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Establishment and identification of the rat knee joint PTJC model. (A) Schematic and X-ray of rat knee joint post-traumatic immobilization. (B) Measurement of extension ROM of the affected knee joint at Day 0, 1, 3, 7, and 14 post-modelling. (C) HE and Masson staining of the posterior joint capsule of the affected knee at day 0, 7, and 14 post-modelling. Scale bars, 50 μm. (D) Expression of fibrosis-associated protein (collagen I, collagen II, collagen III, PDGF, and TGF-β1) in the posterior joint capsule at day 0 and 14 post-modelling were assessed via western blot. Endogenous β-actin was used as an internal control. Error bars represent standard deviation. *P <0.05 compared with the day 0 group.