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. 2021 Feb 17;13(4):5804–5823. doi: 10.18632/aging.202505

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Copyright: © 2021 Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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MIF promoted the proliferation and migration of JFs in vitro. (A, C) JFs were treated with 0–2.5 μg/mL recombinant MIF for 24 h before testing cell proliferation using an EdU assay (A) and cell migration via a Transwell assay (C). (B, D) JFs were treated with 2 μg/mL recombinant MIF combined with 50 μM 4-IPP for 24 h followed by EdU assay (B) and Transwell assay (D). Scale bar, 50 μm. Error bars represent standard deviation. *P <0.05 compared with the 0 μg/mL or DMSO group. #P <0.05 compared with the MIF group.