FIGURE 4.
Effect of CT on membrane potential of B. subtilis 168 (A,C) and S. aureus ATCC 43300 (B,D). (A,B) Membrane potential estimation by flow cytometry using DiOC2(3) dye; Representative data from three independent cultures of both strains were shown following exposure to different agents for 10 min. (C,D) Fluorescence of DiOC2(3) was detected at an excitation wavelength of 485 nm and two emission wavelengths, 530 nm (green) and 630 nm (red), using a microplate reader. Addition of CT to the cell suspensions of bacteria is indicated by an arrow. Data are presented as mean ± SD of three independent experiments. DMSO (1.6%), non-drug treated control; CCCP, depolarization control; Nigericin, hyperpolarization control; VAN (vancomycin), negative control.