Table 4.
Methodology assessment of iodine bactericidal efficacy on PAO1 cultured for 0–3 days on porcine explants (without antibiotic pre‐treatment)*
| A | Exposure (hours) | Biofilm maturity (days) | No. of explants | Growth† |
|---|---|---|---|---|
| Iodoflex™ | 1 | 0 | 3 | Y |
| 12 | 0 | 3 | N | |
| 24 | 0 | 3 | N | |
| 12 | 1 | 3 | Y | |
| 24 | 1 | 3 | N | |
| 12 | 2 | 3 | Y | |
| 24 | 2 | 6 | N | |
| 1 | 3 | 3 | Y | |
| 12 | 3 | 3 | N | |
| 24 | 3 | 6 | N | |
| 72 | 3 | 3 | N | |
| PVP‐I | 1 | 0 | 3 | Y |
| 12 | 0 | 3 | N | |
| 24 | 0 | 3 | N | |
| 12 | 1 | 3 | Y | |
| 24 | 1 | 3 | Y | |
| 12 | 2 | 3 | Y | |
| 24 | 2 | 6 | Y | |
| 1 | 3 | 3 | Y | |
| 12 | 3 | 3 | Y | |
| 24 | 3 | 6 | Y | |
| 72 | 3 | 3 | Y | |
| B | Exposure (hours) | Biofilm maturity (days) | No. of explants | Growth |
| Iodoflex | 24 | 3 | 7 | N |
| 24 | 3 | 2 | Y+ | |
| PVP‐I | 24 | 3 | 9 | Y++ |
*
To determine if cadexomer iodine bacterial load reduction reflects complete kill or residual bactericidal activity after dilution. (A) Single application treated explants incubated for 24 hours at 37°C in 5 ml TSB; explant inoculum: ˜106 CFU (0‐day biofilm). (B) Single application treated explants incubated for 24 hours at 37°C in 5 ml TSB with 100 mg/ml sodium thiosulphate to neutralise iodine.
†
Growth indicated by turbidity of overnight culture (the same outcome was observed for each explant).