FIG 7.

Relative changes in gene expression in PA14 WT and ΔrclR strains in the presence of HOCl or HOSCN. Log₂ fold change of gene expression was plotted against the gene locus tag in order of genomic location. (A) Effect of HOCl on P. aeruginosa gene expression, WT plus HOCl versus WT untreated. (B) Effect of HOSCN on P. aeruginosa gene expression, WT plus HOSCN versus WT untreated. (C) Identification of genes regulated by RclR in the presence of HOCl, WT plus HOCl versus ΔrclR strain plus HOCl. (D) Identification of genes regulated by RclR in the presence of HOSCN, WT plus HOSCN versus ΔrclR strain plus HOSCN. Lines indicate cutoff values at 1.5 or −1.5 log₂ fold change. (A and B) Highlighted upregulated genes include rclX, hypothetical operon (PA14_21570-PA14_21580-PA14_21590-PA14_21600), sulfur transport genes (ssuD-PA14_19570-ssuB), taurine transport genes (PA14_12920-PA14_12940-PA14_12960), drug efflux pump operon mexEF-oprN, pyeRM-xenB operon (HOCl), and T3SS genes. Downregulated genes include pyeRM (HOSCN). (C and D) In these plots, positive log₂ fold change values indicate genes positively regulated by RclR, and negative log₂ fold change values indicate genes negatively regulated by RclR. Highlighted genes positively regulated include rclX, pyocyanin biosynthesis operons phz1 and phz2, denitrification nir and nar operons (HOSCN), and pyeRM (HOCl). Negatively regulated genes include pyeRM-xenB (HOSCN), mexEF-oprN, T3SS genes (HOSCN), and heat shock protein ibpA (HOSCN).