. 2020 Dec 18;203(2):e00300-20. doi: 10.1128/JB.00300-20

TABLE 1.

HOCl-sensitive regulatory gene mutants

PA14 locus tag and gene name^a	PAO1 locus tag	Protein description	P value for mutant OD₆₀₀ vs WT OD₆₀₀ at set time points^b
PA14 locus tag and gene name^a	PAO1 locus tag	Protein description	20 h	22 h	24 h
PA14_07110 sahR	PA0547	ArsR family transcriptional regulator	0.0420	0.0003	<0.0001
PA14_08780 rpoC	PA4269	DNA-directed RNA polymerase β chain	0.0280	0.0002	<0.0001
PA14_13660 tpbA	PA3885	Protein tyrosine phosphatase	0.0522	0.0006	0.0005
PA14_17900 metR	PA3587	LysR family transcriptional regulator	0.0342	0.0002	<0.0001
PA14_20730 flgM (1)	PA3351	Flagellar biosynthesis anti-sigma-28 factor	0.0288	0.0002	<0.0001
PA14_20730 flgM (2)	PA3351	Flagellar biosynthesis anti-sigma-28 factor	0.0299	0.0001	<0.0001
PA14_22370 (2)	PA3233	Hypothetical protein, cyclic nucleotide-binding domain	0.0274	0.0002	<0.0001
PA14_29740	PA2656	Two-component sensor	0.2349	0.0427	0.0214
PA14_32410 mexT	PA2492	LysR family transcriptional regulator of multidrug efflux	0.0370	0.0002	<0.0001
PA14_45950 rsaL	PA1431	Regulatory protein	0.1666	0.0367	0.0385
PA14_52570 rsmA	PA0905	Posttranscriptional global regulator	0.0340	0.0003	<0.0001
PA14_56620 pyeR (2)	PA4354	ArsR family transcriptional regulator	0.0396	0.0010	0.0068
PA14_60860 nfxB	PA4600	TetR family transcriptional regulator of multidrug efflux	0.0515	0.0049	0.0235
PA14_62490 dksA	PA4723	RNA polymerase-binding transcription factor	0.0088	0.0006	0.0018
PA14_68680 envZ (3)	PA5199	Two-component sensor	0.0494	0.0082	0.0714
PA14_70570 recG^c	PA5345	ATP-dependent DNA helicase	0.0053	<0.0001	<0.0001

Mutants were identified as HOCl sensitive by visual inspection (increased lag of >3 h compared to the WT) and statistical analysis of growth. The numbers in parentheses next to some of the gene mutants correspond to genes that have more than one mutant strain in the transposon library (Table S1).

Statistical analysis performed on two biological replicates. P values were determined by one-way analysis of variance with Dunnett’s multiple-comparison test.

recG is not a regulatory mutant per se but is on the same operon as oxyR, which encodes the H₂O₂-sensing transcriptional regulator; the transposon mutant in oxyR was not recoverable.