TABLE 2.

HOCl-resistant regulatory gene mutants

PA14 locus tag and gene namea	PAO1 locus tag	Description	P value for mutant OD600 vs WT OD600 at set time pointsb
			12 h	14 h	16 h	18 h
PA14_06950 (1)	PA0533	LuxR family transcriptional regulator	<0.0001	<0.0001	<0.0001	<0.0001
PA14_09760	PA4185	GntR family transcriptional regulator	0.9538	0.0098	0.0170	0.2154
PA14_10800 ampR	PA4109	LysR family transcriptional regulator of β-lactamase	<0.0001	<0.0001	<0.0001	0.0003
PA14_27900	PA2802	GntR family transcriptional regulator	0.1181	0.0040	0.0040	0.0442
PA14_38040 cmrA	PA2047	AraC family transcriptional regulator	<0.0001	<0.0001	<0.0001	<0.0001
PA14_44490 anr	PA1544	Transcriptional regulator of anaerobic metabolism	<0.0001	<0.0001	<0.0001	0.0053
PA14_50180 fleR (1)	PA1099	Two-component response regulator of flagellar biosynthesis	0.9990	0.2376	0.0471	0.3638
PA14_50200 fleS (2)	PA1098	Two-component sensor of flagellar biosynthesis	0.0610	<0.0001	0.0017	0.0481
PA14_56620 pyeR (1)	PA4354	ArsR family transcriptional regulator	<0.0001	<0.0001	<0.0001	0.0001
PA14_62530 cbrA (1)	PA4725	Two-component sensor of catabolic pathways	<0.0001	<0.0001	<0.0001	0.0398
PA14_62530 cbrA (2)	PA4725	Two-component sensor of catabolic pathways	0.8620	0.0482	0.0405	0.3687
PA14_62540 cbrB	PA4726	Two-component response regulator of catabolic pathways	0.0049	0.0003	0.0140	0.2098
PA14_68680 envZ (1)	PA5199	Two-component sensor	<0.0001	<0.0001	<0.0001	0.0015

^aMutants were identified as HOCl resistant by visual inspection (decreased lag of >3 h compared to the WT) and statistical analysis of growth. The numbers in parentheses next to some of the gene mutants correspond to genes that have more than one mutant strain in the transposon library (Table S1).

^bStatistical analysis performed on two biological replicates. P values were determined by one-way analysis of variance with Dunnett’s multiple-comparison test.