TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source |
|---|---|---|
| Streptococcus mutans | ||
| UA159 | Wild type | |
| ΔSMU.1703c mutant | ΔSMU.1703c::Kmr | This study |
| ΔSMU.1702c mutant | ΔSMU.1702c::Kmr | This study |
| ΔSMU.1703c ΔSMU.1702c mutant | ΔSMU.1703c ΔSMU.1702c::Kmr | This study |
| Bacillus subtilis ΔribB ΔribU mutant | ΔribB::Ermr ΔribU::Kmr | 40 |
| Escherichia coli DH5α | fhuA2 lacΔU169 phoA glnV44 ϕ80' lacZΔM15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | 81 |
| Plasmids | ||
| pHT01 | E. coli to B. subtilis shuttle vector, IPTG-inducible promoter upstream of MCS; Ampr Cmr | MoBiTec GmbH |
| pHT01_1703c | pHT01 with SMU.1703c under control of inducible IPTG promoter | This study |
| pALH124 | Source of Kmr cassette for generating S. mutans mutants | 72 |