TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| S. aureus | ||
| RN4220 | Restriction-negative laboratory strain | J. Iandolo |
| CYL11481 | Newman saeS(P18L) | 18 |
| JE2 | USA300 strain | NARSA |
| NE1109 | JE2 sigB::bursa | NARSA |
| NE1684 | arlR::bursa | NARSA |
| CYLA273 | CYL11481 ΔmgrA::cat | This study |
| CYLA543 | CYL11481 ΔmgrA::ermC | This study |
| CYLA695 | CYL11481 sigB::bursa | This study |
| CYLA696 | CYL11481 ΔmgrA::cat sigB:: bursa | This study |
| CYLA270 | JE2 ΔmgrA::cat | 48 |
| CYLA687 | JE2 sigB::bursa | This study |
| CYLA226 | Newman RNAIII::ermC | This study |
| CYLA230 | CYL11481 RNAIII::ermC | This study |
| CYLA546 | CYL11481 arlR::bursa | This study |
| AL2530 | RN6390 ΔmgrA::ermC | 22 |
| E. coli | ||
| XL1-Blue | Host strain | Strategene |
| CYL2961 | BL21(λDE3)(pLysS)(pTL2961) | This study |
| Plasmids | ||
| pML100 | Shuttle vector, with Pxyl/tetO | 49 |
| pLI50 | Shuttle vector | 50 |
| pET15b | E. coli His tag protein expression vector | Novagen |
| pMLE195 | pML100 with sigB | This study |
| pMLE197 | pML100 with mgrA | This study |
| pMLE57 | pLI50 with sfgfp | This study |
| pTL2961 | pET15b with mgrA | This study |
| pCM11 | sfgfp expression vector | 51 |
| pCL3292 | pCL15 with mgrA | 24 |
| pMLE200 | pMLE57-Pcap(−344 to −78) | This study |
| pMLE201 | pMLE57-Pcap(−344 to −103) | This study |