Figure 9.

Photomicrograph of sulphur mustard (SM) (5 mg/kg) treated mouse skin sections taken at various time intervals, haematoxylin and eosin (H&E). Bar = 25 µm. (A) 1 hour showing ballooning of epidermal cells, acanthosis in epidermis (arrow), oedema of dermis and a few transmigrated neutrophils. (B) 3 hours showing hyperplasia of epidermal cells (arrow), and oedema of dermis along with mild inflammatory reaction. (C) 6 hours showing ballooning of epidermal cells, dermoepidermal seperation (arrow head) and infiltration of inflammatory cells in dermis. (D) 12 hours showing pyknosis of nuclei in basal cells (arrow), dermoepidermal separation (arrow head), oedema and transmigrated inflammatory cells in dermis. (E) 72 hours showing necrosis of epidermis and dermis and necrosed inflammatory cells entrapped in fibrinous exudates (arrow head) forming the eschar tissue. (F) 168 hours showing eschar tissue (arrow) covering the ulcer, coagulative necrosis of epidermis and dermis with atrophied adnexa and remnants of necrosed transmigrated inflammatory cells in dermis.