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Western blot analysis of xanthine oxidase (XO) in wound fluid. The presence of XO in seven wound fluid samples with PUSH scores of 7, 8, 9, 9, 12, 15 and 15, respectively (Lanes 1–7) was determined using Western blotting. Homogenate of bovine liver (5 µg) was used a positive control (Lane 8) and a blank was run in Lane 9 as a negative control. Bands were detected in each sample at approximately 130 kDa.
