Table 1.
Inhibitory activity of OFM and ORC/C extracts against tissue proteases *
| Protease | OFM IC50 (µg/ml) | ORC/C IC50 (µg/ml) |
|---|---|---|
| MMP1 (Collagenase 1) (0·153 U/µl) | 115 ± 14 | ∼600 |
| MMP8 (Collagenase 2) (0·018 U/µl) | 86 ± 2 | ∼600 |
| MMP13 (Collagenase 3) (0·014 U/µl) | 38 ± 2 | ∼600 |
| MMP3 (Stromelysin‐1) (0·029 U/µl) | 96 ± 8 | >600 |
| MMP10 (Stromelysin‐2) (0·01 U/µl) | 45 ± 1 | 355 ± 51 |
| MMP2 (Gelatinase‐A) (0·009 U/µl) | 23 ± 5 | 31 ± 6 |
| MMP9 (Gelatinase‐B) (0·009 U/µl) | 50 ± 14 | 55 ± 7 |
| MMP12 (Macrophage metalloelastase) (0·007 U/µl) | 24 ± 1 | ∼600 |
| MMP14 (Membrane type 1 MMP) (0·024 U/µl) | 45 ± 3 | ∼600 |
| Neutrophil elastase (0·002 mU/µl) † | 157 ± 37 | ∼1740 |
MMP, matrix metalloproteinase; OFM, ovine forestomach matrix; ORC/C, oxidised regenerated cellulose/collagen.
*Errors represent standard errors from triplicate experiments. Concentration in brackets indicates the enzyme concentration used in the respective assay.
†Errors represent standard errors from duplicate experiments.