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. 2021 Feb 25;11:540134. doi: 10.3389/fonc.2021.540134

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Copyright © 2021 Gallegos, Patel, Llopis, Walker, Davidson, Zhang, Zhang and Tilghman

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western Blot analysis of midasin expression. Midasin expression in letrozole-sensitive (AC-1) and letrozole-resistant (LTLT-Ca) breast cancer cells cultured adherently (2D) or as mammospheres (3D). All cells were evaluated by immunoblot to examine the expression of midasin and GAPDH (loading control). (A) Graphs depict normalized percentages of protein expression intensities relative to 2D cell counterparts. (B) Representative immunoblot depicts the protein expression of midasin and GAPDH.