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. 2021 Feb 25;11:625207. doi: 10.3389/fonc.2021.625207

Figure 4.

Figure 4

BGA002 blocks CD276 and restores neuroblastoma (NB) susceptibility to natural killer (NK) cells. (A) Pathway enrichment for four select immune pathways [Gene Ontology (GO) terms] associated to NK cells. (NB1: E-MTAB-1781, NB2: TARGET NB). Symbol size and color intensity indicate—log10 FDR and NES. GO terms enriched in MYCN (left) anti-correlated genes and GO terms enriched in non-MYCN amplification (MNA) (right) patients. (B) mRNA expression inhibition of different genes (MYCN, CD276, CD274, HMGA1, PVR) in NB cell lines measured through real-time PCR after 12 h of treatment with BGA002 2.5 µM (black is the control, red the treatment, n = 4 biological replicates for each cell line). Wilcoxon matched pair test; *P < 0.05, **P < 0.01, where not shown is not significant (P > 0.05). (C) Kelly-luc cell line (MNA NB cell line transfected with luciferase) viability after treatment with BGA002 2.5 µM and NK co-culture (five independent experiments). Wilcoxon matched pair test; **P < 0.01.