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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Exp Hematol. 2020 Sep 8;90:39–45.e3. doi: 10.1016/j.exphem.2020.09.001

Figure 1: Deletion of Flk2 and/or IL7Rɑ differentially affects numbers of both lymphoid and myeloid cells in the lungs of adult mice.

Figure 1:

Cellularity was calculated by the total numbers of cells collected and dividing by the weight of the tissue. Dots represent individual mice. Black bars represent the average. *P<0.05, **P<0.005, ***P<0.001.

A, B lymphocytes were significantly reduced in Il7Rα−/− and FIDKO, but not Flk2−/−, lungs. Quantification of lymphocytes (Live, Ter119-Mac1-Gr1-CD19+) per gram of tissue in the lungs of wild type (WT) (black), Flk2−/− (gray), IL7Rα−/− (white), or FIDKO (red) adult mouse lungs. WT n=8, Flk2−/− n=8, IL7R−/− n=8, and FIDKO n=5 representing four independent experiments.

B, Neutrophils were not affected by loss of Flk2 or IL7Rα. Quantification of neutrophils (Live, CD3-CD4-CD5-CD8-B220-Ter119-CD45+Ly6g+CD11b+) per gram of tissue in the lungs of wild type (WT) (black), Flk2−/− (gray), IL7Rα−/− (white), or FIDKO (red) adult mouse lungs. WT n=8, Flk2−/− n=7, IL7R−/− n=8, and FIDKO n=6 representing four independent experiments.

C, Eosinophils were significantly reduced in Flk2−/−, IL7Rα−/−, and FIDKO mouse lungs. Quantification of eosinophils (Live,CD3-CD4-CD5-CD8-B220-Ter119-CD45+Ly6g-CD11b+SiglecFmidCD11c-) per gram of tissue in the lungs of wild type (WT) (black), Flk2−/− (gray), IL7Rα−/− (white), or Flk2−/−IL7Rα−/− (FIDKO) (red) adult mouse lungs. WT n=6, Flk2−/− n=5, IL7R−/− n=5, and FIDKO n=5 representing four independent experiments.