Fig 3. Enhanced protection against Mtb HN878 in CB6F1 mice with liposomal formulations.

(A) Increased polyfunctional ID93-specific CD4+CD44+ T cells from the spleens of immunized CB6F1 mice four weeks after the final immunization. The data are represented as the percentage of CD4+CD44+CD154+ T cells producing one or more cytokines following ex-vivo stimulation with ID93; cytokine producing subsets are shown as stacked bars, with mean + SD of each subset. Comparisons of total cytokine producing cells between groups were performed using one-way ANOVA with Bonferroni’s multiple comparison test. Down-inflected lines indicate *p<0.05 versus ID93+GLA-LSQ2 or ID93+GLA-LSQ3. (B) Four weeks after LDA infection with Mtb HN878, lungs were taken, and bacterial burden was determined. Bacterial load within the lung is represented as Log₁₀ colony forming units (CFU), with individual mice and mean +/- SD shown. Comparisons among groups were performed using one-way ANOVA with Bonferroni’s multiple comparison test, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 versus Saline; ^p<0.05, ^^p<0.01, ^^^p<0.001 versus BCG.