Fig 6. Prophylactic protection with ID93+GLA-SE and ID93+GLA-LSQ in C57BL/6 mice.

Mice were immunized with ID93 combined with different adjuvant formulations including SE, LS (liposomes), LSQ (liposomes+QS21), GLA-LS, GLA-LSQ, or GLA-SE. (A) The percent frequency of single-cytokine producing ID93-specific CD4+CD44+ T cells. Bars represent the mean of the group, with vertical line indicating SD. Groups were compared for each cytokine using one-way ANOVA with Bonferroni’s multiple comparison test, with *p<0.05, ****p<0.0001 versus ID93 alone; (B) percent of polyfunctional ID93-specific CD4+CD44+CD154+ T cells producing one or more cytokines; cytokine producing subsets are shown as stacked bars, with mean + SD of each subset. Comparisons between groups were performed using one-way ANOVA with Bonferroni’s multiple comparison test, *p<0.05, ****p<0.0001; (C) bacterial burden in the lung is represented as Log₁₀ colony forming units (CFU) 3 weeks after challenge with Mtb H37Rv, with individual mice and mean +/- SD shown. Comparisons between groups were performed using one-way ANOVA with Bonferroni’s multiple comparison test, *p<0.05, **p<0.01, ****p<0.0001 versus Saline; ^p<0.05, ^^p<0.01, ^^^^p<0.0001 versus ID93.