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. 2021 Mar 11;16(3):e0231068. doi: 10.1371/journal.pone.0231068

Table 6. Pre-weaning hematological parameters of control heifers (CON) and heifers supplemented with a blend of essential oils blend (BEO) in milk replacer during pre-weaning.

Item1 Treatment SEM P–value4
CON2 (n = 15) BEO3 (n = 14) T W T x W
RBC (x 106/μL) 8.02 7.95 0.88 0.86 0.63 0.87
PCV (%) 35.53 35.21 5.05 0.85 0.11 0.69
Hb (q/dL) 11.07 10.94 1.61 0.81 0.14 0.73
MCV (fL) 44.74 44.51 2.94 0.74 <0.001 0.51
MCHC (%) 31.10 31.14 0.76 0.87 0.15 0.99
Total leukocytes (/μL) 10,908.45 11,200.78 2,630.0 0.76 0.19 0.22
Basophils (/μL) 2.14 0.00 1.03 <0.001 <0.001 <0.001
Eosinophils (/μL) 68.40 143.90 0.66 0.24 <0.001 0.36
Band neutrophil (/μL) 31.76 26.22 5.69 0.68 0.83 0.31
Segmented neutrophils (/μL) 5,300.63 5,286.56 1,700.0 0.98 <0.001 0.78
Lymphocytes (/μL) 4,837.40 5,082.82 1,120.0 0.66 <0.001 0.01
Monocytes (/μL) 421.60 466.00 247.0 0.48 0.01 0.29
Platelet (x 103/μL) 410.41 353.70 108.0 0.04 <0.001 0.10
Plasmatic protein (g/dL) 6.03 6.03 0.72 1.00 0.17 0.40
PLR 0.08 0.08 0.03 0.91 0.02 0.04
NLR 1.26 1.46 0.03 0.60 <0.001 0.55

1RBC: red blood cell, PCV: packed cell volume, Hb: hemoglobin, MCV: mean corpuscular volume, MCHC: mean corpuscular hemoglobin concentration, PLR: platelet lymphocyte ratio, NLR: neutrophils lymphocytes ratio.

2CON = control

3BEO = 1 g/calf/d blend of essential oil.

4T = treatment effect; W = week effect; T x W = treatment by week interactions.